利用Dualex葉綠素儀研究鷹嘴豆的耐熱性

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測(cè)量?jī)x

在氣候變化的背景下，鷹嘴豆（Cicer arietinum L.）繁殖期的熱應(yīng)激導(dǎo)致了顯著的產(chǎn)量損失。為了確定與熱應(yīng)激耐受性相關(guān)的基因組區(qū)域，使用測(cè)序基因分型方法對(duì)源自DCP 92-3（熱敏感）和ICCV 92944（熱耐受）的重組自交系群體進(jìn)行基因分型，并在正常和晚播或熱應(yīng)激環(huán)境下連續(xù)兩年（2017年和2018年）進(jìn)行評(píng)估。構(gòu)建了包含788個(gè)單核苷酸多態(tài)性標(biāo)記的高密度遺傳圖譜，標(biāo)記跨度為1125cm。利用復(fù)合區(qū)間作圖，13個(gè)性狀中的12個(gè)共鑒定出77個(gè)qtl（37個(gè)主效qtl和40個(gè)副效qtl）。CaLG07上的一個(gè)基因組區(qū)域包含數(shù)量性狀位點(diǎn)（QTL），解釋了從結(jié)莢到結(jié)莢的天數(shù)、100粒重的表型變異>30%，解釋了>10%PVE的氮平衡指數(shù)。此外，我們還報(bào)道了性狀（葉綠素含量、氮平衡指數(shù)、歸一化差異營(yíng)養(yǎng)指數(shù)和細(xì)胞膜穩(wěn)定性等生理性狀）的主要QTL。此外，QTL區(qū)域中編碼熱休克蛋白基因、熱休克轉(zhuǎn)錄因子的32個(gè)候選基因與花粉特異性基因以及開(kāi)花時(shí)間調(diào)控有關(guān)。本研究報(bào)告的主要QTL經(jīng)驗(yàn)證后，可能有助于開(kāi)發(fā)耐熱優(yōu)良品系或品種的分子育種。

關(guān)鍵詞：鷹嘴豆、熱應(yīng)激、測(cè)序基因分型、歸一化差異植被指數(shù)、結(jié)莢天數(shù)

Major QTLs and Potential Candidate Genes for Heat Stress Tolerance Identified in Chickpea (Cicer arietinum L.) 

In the context of climate change, heat stress during the reproductive stages of chickpea (Cicer arietinum L.) leads to significant yield losses. In order to identify the genomic regions responsible for heat stress tolerance, a recombinant inbred line population derived from DCP 92-3 (heat sensitive) and ICCV 92944 (heat tolerant) was genotyped using the genotyping-by-sequencing approach and evaluated for two consecutive years (2017 and 2018) under normal and late sown or heat stress environments. A high-density genetic map comprising 788 single-nucleotide polymorphism markers spanning 1,125 cM was constructed. Using composite interval mapping, a total of 77 QTLs (37 major and 40 minor) were identified for 12 of 13 traits. A genomic region on CaLG07 harbors quantitative trait loci (QTLs) explaining >30% phenotypic variation for days to pod initiation, 100 seed weight, and for nitrogen balance index explaining >10% PVE. In addition, we also reported for the first time major QTLs for proxy traits (physiological traits such as chlorophyll content, nitrogen balance index, normalized difference vegetative index, and cell membrane stability). Furthermore, 32 candidate genes in the QTL regions that encode the heat shock protein genes, heat shock transcription factors, are involved in flowering time regulation as well as pollen-specific genes. The major QTLs reported in this study, after validation, may be useful in molecular breeding for developing heat-tolerant superior lines or varieties.

Keywords: chickpea, heat stress, genotyping-by-sequencing, normalized difference vegetation index, days to pod initiation

相關(guān)閱讀

Dualex 便攜式智能氣象-葉綠素-花青素-黃酮醇測(cè)量?jī)x

PESSL便攜式便攜式植物多酚-葉綠素儀原理

Dualex植物多酚-葉綠素儀：腐殖酸通過(guò)鐵依賴和非鐵依賴的協(xié)同機(jī)制緩解禾本科植物的鐵失綠癥

Dualex植物多酚-葉綠素儀：硝化抑制劑和/或間作效應(yīng)下玉米（和小麥灌溉種植系統(tǒng)中的硝酸鹽淋失

Dualex植物多酚-葉綠素儀：與保持綠色表達(dá)相關(guān)的葉片和冠層性狀與對(duì)水分脅迫具有不同耐性的小麥基因型的產(chǎn)量構(gòu)成密切相關(guān)

Dualex植物多酚-葉綠素儀：基于地面和智能傳感器的冬小麥營(yíng)養(yǎng)營(yíng)養(yǎng)儀

Dualex植物多酚-葉綠素儀：增加角質(zhì)層蠟沉積不會(huì)改變殘余葉片蒸騰作用

Dualex植物多酚-葉綠素儀--氮平衡指數(shù)：不同生物刺激劑對(duì)水培生菜的效果比較

Dualex植物多酚-葉綠素儀：固體消化物和葡萄園冬季修剪堆肥替代苗圃中微繁殖高叢藍(lán)莓生長(zhǎng)基質(zhì)中的泥炭研究

Dualex植物多酚-葉綠素儀：不同灌溉制度下根際細(xì)菌和藻類對(duì)金盞草生理生化指標(biāo)的影響

Dualex植物多酚-葉綠素儀：Sentinel-2A與近端傳感器數(shù)據(jù)協(xié)同提取水稻不同生育期生化參數(shù)